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Anthony Tsikouras
Graduate student, PhD candidate in Engineering Physics
McMaster University - Photonics stream

Email: tsikoua@mcmaster.ca
Skype ID: atsikouras1
Office: ETB/529 | Lab: ETB/431
Office Phone: (905) 525-9140 x26196
Lab Phone: (905) 963-3777
Cell Phone: (905) 902-3897















Research

Fluorescence imaging typically involves tagging components of a sample with matched fluorophores, then measuring the fluorescence intensity of the sample when it is stimulated with an excitation source. Fluorescence lifetime imaging microscopy (FLIM) is a technique whereby the decay time of a fluorescence signal is used, instead of its intensity. While this adds a layer of complexity and cost, it can be a much more reliable method of obtaining information about the the cell's microenvironment. For instance, when a fluorophore is in close proximity to another molecule, Förster resonant energy transfer (FRET) occurs. In the FRET process, a fluorophore transfers its energy (which would normally be fluoresced) to the nearby molecule. This acts to quench the fluorescence lifetime, causing it to be shorter than the fluorophore's usual lifetime decay. Measuring the lifetime of the fluorophore can then be an excellent indicator of protein-protein interaction.

I am prototyping a high-throughput high content microscope system that implements FLIM for use in drug discovery systems. The system is being optimized for speed to match high-throughput standards, as well as high spatial and temporal resolution.

Publications

Tsikouras, A., Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012) Streak camera crosstalk reduction using a multiple delay optical fiber bundle. Optics Letters. 37: 250-252.

Conference Presentations

Tsikouras, A. (2014), “Designing a scanning system for multiplexed confocal FLIM microscopy using refractive window tilting,” Society of Biomolecular Imaging and Informatics 1. Tsikouras, A., Yeh, S.C., Fang, Q. (2014), “Instrumentation design for high-speed FLIM for applications in high-content screening,” Electrochemical Society Meeting 225, 1487.
Tsikouras, A., Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012), High-throughput FLIM-FRET nanoimaging for quantification of protein-protein interactions in high-content screening [oral presentation]. SPIE Optics + Photonics (conference). 8460-04.

Schedule

Spring 2013 Plan
Summer 2014 Plan
Upcoming Plan
Planning Stage

Monday Tuesday Wednesday Thursday Friday
Site Spectral Sunnybrook Sunnybrook McMaster McMaster
7:30am
8:30am
9:30am
10:30am
11:30am
12:30pm
1:30pm
2:30pm
3:30pm
4:30pm
5:30pm
6:30pm
7:30pm
8:30pm


Courses taken:

Course Description Semester
BME 701 Biomedical Engineering Core I 2010/2011 Term 1
BME 706 Biomedical Engineering Core II 2010/2011 Term 2
ECE 762 Detection and Estimation Theory 2011/2012 Term 1
CSE 701 Foundations of Modern Scientific Programming 2013/2014 Term 1
CE 6TN4 Image Processing 2013/2014 Term 2
BME 707 Advanced Topics in Biophotonics 2014/2015 Term 1


Safety Training

Base CoursesAdditional Courses
CourseDateCourseDate
WHMIS 2009 WHMIS Update 2014/11
Fire Safety 2009 Fire Safety update 2014/11
Biosafety 2 2011 BSL-2 update 2014/11
Laser Safety 2009 Laser Safety Lecture 2010
Asbestos Awareness 2009 Emergency Code Procedures 2013/09
Ergonomics 2009 Transportation of Dangerous Good TBA
Slips/Trips/Falls 2009 Chemical Handling 2010
Eng Phys Safety 2009
Electrical Safety Awareness I 2012

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