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Anthony Tsikouras
Graduate student, M.A.Sc Engineering Physics candidate
McMaster University - Photonics stream

Email: tsikoua@mcmaster.ca
Skype ID: atsikouras1
Office: ETB/529 | Lab: ETB/431
Office Phone: (905) 525-9140 x26196
Lab Phone: (905) 963-3777
Cell Phone: (905) 902-3897















Research

Fluorescence imaging typically involves tagging components of a sample with matched fluorophores, then measuring the fluorescence intensity of the sample when it is stimulated with an excitation source. Fluorescence lifetime imaging microscopy (FLIM) is a technique whereby the decay time of a fluorescence signal is used, instead of its intensity. While this adds a layer of complexity and cost, it can be a much more reliable method of obtaining information about the the cell's microenvironment. For instance, when a fluorophore is in close proximity to another molecule, Förster resonant energy transfer (FRET) occurs. In the FRET process, a fluorophore transfers its energy (which would normally be fluoresced) to the nearby molecule. This acts to quench the fluorescence lifetime, causing it to be shorter than the fluorophore's usual lifetime decay. Measuring the lifetime of the fluorophore can then be an excellent indicator of protein-protein interaction.

I am prototyping a high-throughput high content microscope system that implements FLIM for use in drug discovery systems. The system is being optimized for speed to match high-throughput standards, as well as high spatial and temporal resolution.

Publications

Tsikouras, A., Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012) Streak camera crosstalk reduction using a multiple delay optical fiber bundle. Optics Letters. 37: 250-252.

Conference Presentations

Tsikouras, A., Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012) High-throughput FLIM-FRET nanoimaging for quantification of protein-protein interactions in high-content screening [oral presentation]. SPIE Optics + Photonics (conference). 8460-04.

Schedule

Spring 2013 Plan
Upcoming Plan
Planning Stage

I am on campus 8am-4pm daily in my office (ETB/529), lab (ETB/431) or in the Biophotonics Facility (HSC/4H22).

Monday Tuesday Wednesday Thursday Friday
7:30am
8:30am CSE 701
9:30am CSE 701
10:30am CSE 701
11:30am EP 2W03
12:30pm EP 2W03
1:30pm
2:30pm
3:30pm
4:30pm
5:30pm EP 2W03 EP 2W03
6:30pm
7:30pm
8:30pm


Possible future courses:

Course Description 2013/2014
BIOCHEM 6H03 Biotechnology and Drug Discovery Term 2
BIOCHEM 711 Special Topics in Microscopy and Photonics Not Offered
CAS 6TI3 Fundamentals of Image Processing Not Offered
CAS 750 Model-Based Image Reconstruction Not Offered
CAS 755 Advanced Computational Methods and Models Term 2
CHEM 6C03 Statistics for Engineers Term 2
COMP ENG 6TN4 Image Processing Term 2
CSE 701 Foundations of Modern Scientific Programming Term 1
ECE 710 Engineering Optimization Term 2
ECE 733 Non-Linear Optimization for Electrical Engineers Term 2
ECE 771 Algorithms for Parameter and State Estimation Term 1
ENG PHYS 726 Optoelectronic Device Physics Term 2
ENG PHYS 734 Nonlinear Optics Not Offered
MOL BIOL 6H03 Molecular Biology of Cancer Term 2


Safety Training

Base CoursesAdditional Courses
CourseDateCourseDate
WHMIS 2009, Sept. 2013 Laser Safety 2009
Fire Safety 2009, Sept. 2013 Chemical Handling 2010
Asbestos Awareness 2009 Biosafety BSL-2 2011, Sept. 2013
Ergonomics 2009 Emergency Code Procedures Sept. 2013
Slips/Trips/Falls 2009 Transportation of Dangerous Good TBA
Eng Phys Safety 2009
Electrical Safety Awareness I 2012

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