2010

Monday May 3

Lec 1 - Introduction&LabRecords (H.E. Schellhorn)

Lec 2 - Proteins&SDS (H.E. Schellhorn)

Tuesday May 4

Lec 1 - DNA primer design and software (T.Dong)

Lec 2 - PAGE separation (S. Chiang)

Wednesday May 5

Lec 1 - CellFractionation (H.E Schellhorn)

Lec 2 - SpectroscopyandEnzymeAssays (H.E Schellhorn)

Thursday May 6

Lec 1 - Protein&EnzymeAssays (H.E. Schellhorn)

Lec 2 - Immuno (Western) Blotting (S. Sathiasothy)

Friday May 7

Lec 1 - Scanning Electron Microscopy (Klaus Schultes)

Lec 2 - Cell Culturing Techniques (Alison Cowie)

Monday May 10

Rotation exercises– HPLC, EM, PCR, Microtiter plate, autoclave (no notes)

Tuesday May 11

Rotation exercises completion and bacterial growth curve for induction of Taq

Wednesday May 12

Lec 1 - DNA synthesis and PCR (Alison Cowie)

Thursday May 13

Lec 1 - Immunofluorescence and Fluorescent Proteins (H.E. Schellhorn)

2009 Lecture Notes

(these are maintained for students who wished to gain an overview of the course–lectures will be updated with changes (usually minor) on a daily basis as the course progresses in 2010).

2009

Monday, May 4

Introduction (H. Schellhorn)

Primer design using Vector NTI (T. Dong)

Lab records (H. Schellhorn)

Tuesday, May 5

Protein Over Expression (Sigma Factors) (H. Schellhorn)

Polyacrylamide Gel Electrophoresis (H. Schellhorn)

Polyacrylamide Gel Electrophoresis(modified abit) (H. Schellhorn)

Wednesday, May 6

Cell Fractionation (H. Schellhorn)

Spectroscopy Enzyme Assay and Protein (H. Schellhorn)

Thursday, May 7

Immunological Methods (H. Schellhorn)

Western Blotting and Analysis (Sharmila)

Friday May 8

Protein Assays and Calculations (H. Schellhorn)

Scanning Electron Microscopy (Klaus Schultes)

Cell Culture Techniques (Alison Cowie)

Monday May 11

Tuesday May 12

Scientific Method (Dr. R.A. Morton)

Publication Quality Information (H. Schellhorn)

Taq Purification Notes (T. Dong)

Oligo Synthesis and sequencing (A. Cowie)


Personal Tools