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2010
Lec 1 - Introduction&LabRecords (H.E. Schellhorn)
Lec 2 - Proteins&SDS (H.E. Schellhorn)
Lec 1 - DNA primer design and software (T.Dong)
Lec 2 - PAGE separation (S. Chiang)
Lec 1 - CellFractionation (H.E Schellhorn)
Lec 2 - SpectroscopyandEnzymeAssays (H.E Schellhorn)
Lec 1 - Protein&EnzymeAssays (H.E. Schellhorn)
Lec 2 - Immuno (Western) Blotting (S. Sathiasothy)
Lec 1 - Scanning Electron Microscopy (Klaus Schultes)
Lec 2 - Cell Culturing Techniques (Alison Cowie)
Rotation exercises– HPLC, EM, PCR, Microtiter plate, autoclave (no notes)
Rotation exercises completion and bacterial growth curve for induction of Taq
Lec 1 - DNA synthesis and PCR (Alison Cowie)
Lec 1 - Immunofluorescence and Fluorescent Proteins (H.E. Schellhorn)
2009 Lecture Notes
(these are maintained for students who wished to gain an overview of the course–lectures will be updated with changes (usually minor) on a daily basis as the course progresses in 2010).
2009
Introduction (H. Schellhorn)
Primer design using Vector NTI (T. Dong)
Lab records (H. Schellhorn)
Protein Over Expression (Sigma Factors) (H. Schellhorn)
Polyacrylamide Gel Electrophoresis (H. Schellhorn)
Polyacrylamide Gel Electrophoresis(modified abit) (H. Schellhorn)
Cell Fractionation (H. Schellhorn)
Spectroscopy Enzyme Assay and Protein (H. Schellhorn)
Immunological Methods (H. Schellhorn)
Western Blotting and Analysis (Sharmila)
Protein Assays and Calculations (H. Schellhorn)
Scanning Electron Microscopy (Klaus Schultes)
Cell Culture Techniques (Alison Cowie)
Monday May 11
Tuesday May 12
Scientific Method (Dr. R.A. Morton)
Publication Quality Information (H. Schellhorn)
Taq Purification Notes (T. Dong)
Oligo Synthesis and sequencing (A. Cowie)
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