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people:tsikourasa:planningstage 2013/06/18 13:56 people:tsikourasa:planningstage 2013/06/19 12:52 current
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While these two tasks have been done independently, they have not been performed simultaneously in a real-time scheme. The combination of the two fix the phase and determine the sweep amplitude, allowing for a full and accurate mapping of the non-uniform time axis independently for each frame. While these two tasks have been done independently, they have not been performed simultaneously in a real-time scheme. The combination of the two fix the phase and determine the sweep amplitude, allowing for a full and accurate mapping of the non-uniform time axis independently for each frame.
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=== Validation Experiment === === Validation Experiment ===
 +
 +  - Demonstration of the correct remapping of a fluorescence standard decay (Coumarin 6?) at different points on the screen
 +  - Timelapse demonstrating how the pulse COG feedback prevents phase drift
 +  - Temporal remapping at different points in time using the changing reference pulse positions to recalculate the scheme
==== Galvo Scanner Optics Letter ==== ==== Galvo Scanner Optics Letter ====
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__Differences:__ __Differences:__
  * application meant for laser-machining   * application meant for laser-machining
 +  * device used to scan a single spot, though it is technically capable of multiple foci
**Poland, S. P., Coelho, S., Krstajić, N., Tyndall, D., Walker, R., Li, D. D., Henderson, R., et al. (2013). Development of a fast TCSPC FLIM-FRET imaging system. Proceedings of the SPIE (Vol. 8588, pp. 1–8).** **Poland, S. P., Coelho, S., Krstajić, N., Tyndall, D., Walker, R., Li, D. D., Henderson, R., et al. (2013). Development of a fast TCSPC FLIM-FRET imaging system. Proceedings of the SPIE (Vol. 8588, pp. 1–8).**
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__Differences:__ __Differences:__
  * galvo mirrors used to perform the scan   * galvo mirrors used to perform the scan
 +
 +==Other Types of Scanners==
 +
 +  - **Nipkow-disk** - unsuitable for a static array  of detectors (collection fiber array) \\ (Bewersdorf, J., Pick, R., and Hell, S.W., 1998, Multifocal multi-photon microscopy, Opt. Lett. 23:655–657.)
 +  - **Scanning stage** - unsuitable for high-throughput scanning, biological samples \\ (Nielsen, T., Fricke, M., Hellwig, D., and Andersen, P., 2001, High efficiency beam splitter for multifocal multi-photon microscopy, J. Microsc. 201:368–376.)
=== Novelty of Invention === === Novelty of Invention ===
 +
 +The novelty of the invention is the use of window-equipped galvo scanners as a means of evenly shifting a foci array for use in point-scanning microscopy.
 +
 +While window galvos have been implemently before, this is the first known demonstration that applies them to a) microscopy and b) multiple foci. Typically, multiple foci schemes in microscopy are scanned with the use of traditional mirror galvos, though these do not achieve the same level of uniform foci shifting applied across an entire foci array. They also run galvos at the limits of their achievable resolution, and require sets of relay lenses and potentially specialized scan lenses (f-theta lenses) to provide a linear output.
=== Validation Experiment === === Validation Experiment ===
 +
 +  - Reconstructed image of a raster scan target (Convallaria?) using the window galvo system
 +  - Readout images at the sample stage to quantify the foci array shift vs. window tilt

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