Zhaojun Nie 聂肇君

Graduate student, PhD Candidate. Biomedical Engineering, McMaster University
M.Sc Optoelectronic Engineering, Beijing Institute of Technology
B.S Optical Information Science & Technology, Xi'an Institute of Post and Telecomunication.

Office: ITB 112
Email: niez2@mcmaster.ca

Mailing Address
ETB 405 School of Biomedical Engineering
McMaster University
1280 Main Street West
Hamilton, ON L8S 4L7, Canada

Integrated system of time-resolved fluoresecence spectroscopy and diffuse reflectance spectroscopy for optical biopsy
Abstract:
Time resolved fluorescence (TRF) spectroscopy and diffuse reflectance (DR) spectroscopy have been used as minimally-invasive optical biopsy modalities. They offer real-time alternatives to invasive tissue biopsies. Fluorescence lifetime is independent of intensity variations and adds an additional source of contrast compared to steady state fluorescence. Diffuse reflectance allows for quantitative measurement of optical properties of tissue. Combining both DR and TRF modalities in one optical biopsy instrument allows for the integration of diffuse reflectance, time-resolved, and steady-state spectra for tissue diagnosis as well as real-time correction of the fluorescence lifetime and spectrum based on optical property measurements in-situ. We have designed and developed an integrated clinically compatible apparatus which acquires time-resolved fluorescence and diffuse reflectance simultaneously. We have also designed and fabricated a dual modality, compact fiber-optic probe for fluorescence and diffuse reflectance signal collection. The performance of the system was evaluated and calibrated using a fluorescence tissue phantom model.
Scanning time resolved fluorescence imaging system
Abstract:

Time-resolved fluorescence and diffuse reflectance spectroscopy study in ex-vivo brain tissue
Abstract:

Time-Resolved Fluorescence Based Optical Biopsy in the UGI Tract: A Pilot ex vivo Study
Abstract:
Screening and surveillance for gastrointestinal (GI) cancers by endoscopic biopsy is invasive, costly, and has the potential for sampling error. Cancer surveillance, as in Barrett’s esophagus, is particularly challenging because of the surface areas of disease involvement. Endogenous fluorescence of mucosal tissueprovides intrinsic biochemical and physiological information that may enable real-time, functional diagnosis when combined with white-light endoscopy. Time-resolved fluorescence (TRF) spectroscopy characterizestissue fluorescence emission in terms of intensity, spectral distribution, and radioactive lifetime (Figures 1). Therefore, TRF techniques are attractiveforin vivo minimally-invasive optical biopsy applications, which may provide a more efficient approach to improve the sensitivity of(needle)biopsy while reducing the number of biopsies required, increasing surveillance intervals, and reducing cost.
Fluorescence lifetime fitting method
Abstract: (software package is available )

Zhaojun Nie, Derek Capon, Joseph E. Hayward, Thomas Farrell, and Qiyin Fang,”Time-Resolved Fluorescence based optical biopsy technology development”
Conference :Thanking Ahead For a Strong Future Canada Research Chairs 2010

F. Tse, L. Liu, D. Armstrong, Z. Nie, M. LePalud, T. Mursalin, M. J. Deen, Q. Fang.”Time-Resolved Fluorescence Based Optical Biopsy in the UGI Tract: A Pilot ex vivo Study” 2011.

Derek J. Cappon, Zhaojun Nie, Thomas J. Farrell, Qiyin Fang, and Joseph E. Hayward,”A Novel Optical Property Recovery Algorithm for Use in the Optical Biopsy of Brain Tissue” in Digital Holography and Three-Dimensional Imaging, OSA Technical Digest (Optical Society of America, 2012)

Zhaojun Nie, Derek Cappon, Joseph E. Hayward, Thomas J. Farrell, Michael S. Patterson, John Provias, Naresh Murty, William McMillan, and Qiyin Fang.”Integrated Time-resolved Fluorescence and Diffuse Reflectance Spectroscopy Optical Biopsy Instrument” in BIOS,2011 SPIE photonics west

Zhaojun Nie, Derek Cappon, Joseph E. Hayward, Thomas J. Farrell, Michael S. Patterson, John Provias, Naresh Murty, William McMillan, Brian Pikul, William H. Yong ,and Qiyin Fang.”Integrated Time-resolved Fluorescence and Diffuse Reflectance Spectroscopy Optical Biopsy Instrument” in 9th Annual World Congress of SBMT on Brain, Spinal Cord Mapping and Image Guided Therapy