Goal: Finish FLIM project and finalized the results

Main purpose of the study:
Characterizing time-lapse fluorescence lifetimes of Photofrin at different intracellular locations in order to realize if FLIM can be a potential real-time dose metric for PDT.

5 / 4 - 5 / 10 :
Restart the cell culture and data analysis of (L1_1.5hr to 4 hr)

5 / 11 - 5 / 17 :
Data analysis (Cells are getting happier during these two weeks)

5 / 18 - 5 / 24 :
Start FLIM experiment using SP5

• Low concentration (5ug/ml), 1 hour of incubation (need 2 more replicates)
  
• Data analysis
  

5 / 25 - 5 / 31 :
FLIM experiment (cont'd)

• Low concentration (5ug/ml), 1.5 hr to 4 hr of incubation (need 2 more replicates)
  
• Data analysis
  

6 / 1 - 6 / 10:
FLIM experiment and organize all results (low concentration)

• Low concentration (5ug/ml), 18 hr of incubation – need 2 more replicates
• Data analysis
  

6 / 11 - 6 / 30:
Organize all results using low concentration Photofrin

• Decide what other measurements need to be done (i.e. calibration of IRF..etc) after summarizing the results.
  
• Note: We don't have any repetitive measurement for high concentration photofrin (20 ug/ml), not sure if it's necessary
  

7 / 1 - 7 / 30:
to be arranged based on experimental results and due date

• May start to finalize FLIM project, hopefully will be able to start the manuscript preparation. Angela may perform some leftover experiments.
  

Hopefully working on the manuscript of FLIM project. To be arranged based on the progress.