people:tsikourasa

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**Anthony Tsikouras**\\
Graduate student, M.A.Sc Engineering Physics candidate \\
[[http://engphys.mcmaster.ca|McMaster University]] -  Photonics stream\\ \\
**Email:** [[tsikoua@mcmaster.ca]] \\
**Skype ID:** atsikouras1 \\
**Office:** ETB/529 | **Lab:** ETB/431 \\
**Office Phone:** (905) 525-9140 x26196 \\
**Lab Phone:** (905) 963-3777 \\
**Cell Phone:** (905) 902-3897 \\
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===Research===
Fluorescence imaging typically involves tagging components of a sample with matched fluorophores, then measuring the fluorescence intensity of the sample when it is stimulated with an excitation source.  Fluorescence lifetime imaging microscopy (FLIM) is a technique whereby the decay time of a fluorescence signal is used, instead of its intensity. While this adds a layer of complexity and cost, it can be a much more reliable method of obtaining information about the the cell's microenvironment. For instance, when a fluorophore is in close proximity to another molecule, Förster resonant energy transfer (FRET) occurs. In the FRET process, a fluorophore transfers its energy (which would normally be fluoresced) to the nearby molecule. This acts to quench the fluorescence lifetime, causing it to be shorter than the fluorophore's usual lifetime decay. Measuring the lifetime of the fluorophore can then be an excellent indicator of protein-protein interaction.

I am prototyping a high-throughput high content microscope system that implements FLIM for use in drug discovery systems. The system is being optimized for speed to match high-throughput standards, as well as high spatial and temporal resolution.  \\

===Publications===

**Tsikouras, A.**, Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012) Streak camera crosstalk reduction using a multiple delay optical fiber bundle. Optics Letters. 37: 250-252.

===Conference Presentations===

**Tsikouras, A.**, Ning, J., Ng. S., Sinclair, P., Berman, R., Andrews, D.W., Fang, Q. (2012) High-throughput FLIM-FRET nanoimaging for quantification of protein-protein interactions in high-content screening [oral presentation]. SPIE Optics + Photonics (conference). 8460-04.

===Schedule===

/*[[tsikourasa:summer_2011|Summer Plan]]
[[tsikourasa:fall_2012|Fall 2012 Plan]]*/
[[people:tsikourasa:spring_2013|Spring 2013 Plan]] \\
[[people:tsikourasa:plan|Upcoming Plan]] \\
[[people:tsikourasa:planningstage|Planning Stage]]

I am on campus **8am-4pm** daily in my office (ETB/529), lab (ETB/431) or in the Biophotonics Facility (HSC/4H22).

/*==Upcoming Experiments==

^  Date  ^Experiment  ^
^  July 5 @ 2pm  |Live cell imaging Mito-Tracker  |
^  July 6 @ 10am  |Live cell imaging FRET standards: C5V, C17V, C32V  |*/

^         ^  Monday        ^  Tuesday        ^  Wednesday    ^    Thursday    ^  Friday         ^
^  7:30am   |                |                 |               |                |                 |
^  8:30am   |                |                 ^  CSE 701      |                |                 |
^  9:30am   |                |                 ^  CSE 701      |                |                 |
^  10:30am  |                |                 ^  CSE 701      |                |                 |
^  11:30am  |                |                 |               ^  EP 2W03       |                 |
^  12:30pm  |                |                 |               ^  EP 2W03       |                 |
^  1:30pm   |                |                 |               |                |                 |
^  2:30pm   |                |                 |               |                |                 |
^  3:30pm   |                |                 |               |                |                 |
^  4:30pm   |                |                 |               |                |                 | 
^  5:30pm   ^  EP 2W03       |                 |               ^  EP 2W03       |                 | 
^  6:30pm   |                |                 |               |                |                 | 
^  7:30pm   |                |                 |               |                |                 | 
^  8:30pm   |                |                 |               |                |                 |

\\
Possible future courses:

^Course         ^Description                                       ^  2013/2014    ^
|BIOCHEM 6H03   |Biotechnology and Drug Discovery                  |  Term 2       |
|BIOCHEM 711    |Special Topics in Microscopy and Photonics        |  Not Offered  |
|CAS 6TI3       |Fundamentals of Image Processing                  |  Not Offered  |
|CAS 750        |Model-Based Image Reconstruction                  |  Not Offered  |
|CAS 755        |Advanced Computational Methods and Models         |  Term 2       |
|CHEM 6C03      |Statistics for Engineers                          |  Term 2       |
|COMP ENG 6TN4  |Image Processing                                  |  Term 2       |
|CSE 701        |Foundations of Modern Scientific Programming      |  Term 1       |
|ECE 710        |Engineering Optimization                          |  Term 2       |
|ECE 733        |Non-Linear Optimization for Electrical Engineers  |  Term 2       |
|ECE 771        |Algorithms for Parameter and State Estimation     |  Term 1       |
|ENG PHYS 726   |Optoelectronic Device Physics                     |  Term 2       |
|ENG PHYS 734   |Nonlinear Optics                                  |  Not Offered  |
|MOL BIOL 6H03  |Molecular Biology of Cancer                       |  Term 2       |


\\
===Safety Training===

^Base Courses^^Additional Courses^^
^Course^Date^Course^Date^
|WHMIS  |  2009, Sept. 2013  |Laser Safety  |  2009   |
|Fire Safety  |  2009, Sept. 2013  |Chemical Handling  |  2010  |
|Asbestos Awareness  |  2009  |Biosafety BSL-2  |  2011, Sept. 2013  |
|Ergonomics  |  2009  |Emergency Code Procedures  |  Sept. 2013  |
|Slips/Trips/Falls  |  2009  |Transportation of Dangerous Good  |  TBA  |
|Eng Phys Safety  |  2009  |
|Electrical Safety Awareness I  |  2012  |

===Related Links===

[[project_notes:multi_confocal_flim|Project Research Page]]
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