people:2011_summer_plans

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===== 2011 Summer Plans =====
**Goals:** Finish FLIM project and finalized the results\\
**Main purpose of the study:** \\
Characterizing time-lapse fluorescence lifetimes of Photofrin at different intracellular locations in order to realize if FLIM can be a potential real-time dose metric for PDT.\\

==== May ====
**5/4 - 5/10** :\\ Restart the cell culture and data analysis of (L1_1.5hr to 4 hr)\\
**5/11 - 5/17**: \\data analysis (Cells are getting happier during these two weeks)\\
**5/18 - 5/24**: \\Start FLIM experiment using SP5 --\\
  * Low concentration (5ug/ml), 1 hour of incubation (need 2 more replicates)\\
  * Data analysis\\


**5/25 - 5/31**\\
FLIM experiment (cont'd)--\\
  * Low concentration (5ug/ml), 1.5 hr to 4 hr of incubation (need 2 more replicates)\\
  * Data analysis\\


==== June ====
**6/1 - 6/10**:\\
FLIM experiment and organize all results (low concentration)\\
  * Low concentration (5ug/ml), 18 hr of incubation -- need 2 more replicates
  * Data analysis\\

**6/11 - 6/30**:\\
Organize all results using low concentration Photofrin\\
  * Decide what other measurements need to be done (i.e. calibration of IRF..etc) after summarizing the results.\\
  * Note: __We don't have any repetitive measurement for high concentration photofrin (20 ug/ml), not sure if it's necessary__\\


==== July ====
**7/1 - 7/30**:\\
to be arranged based on experimental results and due date\\
  * May start to finalize FLIM project, hopefully will be able to start the manuscript preparation. Angela may perform some leftover experiments.\\

==== August ====
Hopefully working on the manuscript of FLIM project. To be arranged based on the progress. \\

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