people:2011_summer_plans

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===== 2011 Summer Plans =====
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**Goal:** Finish FLIM project and finalized the results\\
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**Main purpose of the study:** \\
Characterizing time-lapse fluorescence lifetimes of Photofrin at different intracellular locations in order to realize if FLIM can be a potential real-time dose metric for PDT.\\
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=== May ===

**5 / 4 - 5 / 10** :\\ 
Restart the cell culture and data analysis of (L1_1.5hr to 4 hr)\\
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**5 / 11 - 5 / 17** :\\
 Data analysis (Cells are getting happier during these two weeks)\\
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**5 / 18 - 5 / 24** :\\ 
Start FLIM experiment using SP5 \\
  * Low concentration (5ug/ml), 1 hour of incubation (need 2 more replicates)\\
  * Data analysis\\

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**5 / 25 - 5 / 31** :\\
FLIM experiment (cont'd)\\
  * Low concentration (5ug/ml), 1.5 hr to 4 hr of incubation (need 2 more replicates)\\
  * Data analysis\\
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=== June ===
**6 / 1 - 6 / 10**:\\
FLIM experiment and organize all results (low concentration)\\
  * Low concentration (5ug/ml), 18 hr of incubation -- need 2 more replicates
  * Data analysis\\
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**6 / 11 - 6 / 30**:\\
Organize all results using low concentration Photofrin\\
  * Decide what other measurements need to be done (i.e. calibration of IRF..etc) after summarizing the results.\\
  * <hi #ffff00>Note: We don't have any repetitive measurement for high concentration photofrin (20 ug/ml), not sure if it's necessary</hi> \\
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=== July ===
**7 / 1 - 7 / 30**:\\
to be arranged based on experimental results and due date\\
  * May start to finalize FLIM project, hopefully will be able to start the manuscript preparation (i.e. write-up of some results and methods). Angela may perform some leftover experiments.\\
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=== August ===
Hopefully working on the manuscript of FLIM project. To be arranged based on the progress. \\

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