Trace:
Differences
This shows you the differences between the selected revision and the current version of the page.
| protocols-sops 2010/11/24 21:18 | protocols-sops 2010/11/24 21:20 current | ||
|---|---|---|---|
| Line 9: | Line 9: | ||
| Materials & Reagents | Materials & Reagents | ||
| Alkaline lysis solution I: 50mM glucose, 25mM Tris-Cl pH 8.0, 10mM EDTA pH 8.0 | Alkaline lysis solution I: 50mM glucose, 25mM Tris-Cl pH 8.0, 10mM EDTA pH 8.0 | ||
| + | |||
| Alkaline lysis solution II: 0.2N NaOH, 1% SDS; make fresh | Alkaline lysis solution II: 0.2N NaOH, 1% SDS; make fresh | ||
| Alkaline lysis solution III: 60ml 5M potassium acetate, 11.5ml glacial acetic acid, 28.5ml ddH2O; place on ice before use | Alkaline lysis solution III: 60ml 5M potassium acetate, 11.5ml glacial acetic acid, 28.5ml ddH2O; place on ice before use | ||
| Line 40: | Line 41: | ||
| ===== DNA quantification_ using Spectrophotometer ===== | ===== DNA quantification_ using Spectrophotometer ===== | ||
| - | |||
| Materials & Reagents | Materials & Reagents | ||
You are here: start » protocols-sops
