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===== Presentations ===== | ===== Presentations ===== | ||
- | === 2010 Lecture Notes === | + | === 2013 Lecture Notes === |
+ | Monday May 6 | ||
- | **[[Monday May 3]]** | + | |
+ | {{:2013:day_1-1-introduction.pdf}} | ||
- | **{{:2010:day_1-1-introduction.pdf|Lec 1 - Introduction&LabRecords}} (H.E. Schellhorn)** | + | {{:2013:day_1-2_primer_assignment_.pdf|}} |
- | **{{:2010:day_1-2-proteinexpression_.pdf|Lec 2 - Proteins&SDS}} (H.E. Schellhorn)** | + | Note sigma factor notes included in tomorrow's lecture notes. |
- | **[[Tuesday May 4]]** | + | Tuesday May 7 |
- | + | ||
- | **{{:2010:day_2-1-primer_design_using_vector_nti.pdf|Lec 1 - DNA primer design and software}} (T.Dong)** | + | |
- | + | ||
- | **{{:2010:day_2-2-electrophoresis.pdf|Lec 2 - PAGE separation}} (S. Chiang)** | + | |
- | + | ||
- | **[[Wednesday May 5]]** | + | |
- | + | ||
- | **{{:2010:day3-2-cellfractionation.pdf|Lec 1 - CellFractionation}} (H.E Schellhorn)** | + | |
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- | **{{:2010:day_3-2-spectroscopyandenzymeassays.pdf|Lec 2 - SpectroscopyandEnzymeAssays}} (H.E Schellhorn)** | + | |
- | + | ||
- | **[[Thursday May 6]]** | + | |
- | + | ||
- | **{{:2010:day4-1-protein_enzymeassays.pdf|Lec 1 - Protein&EnzymeAssays}} (H.E Schellhorn)** | + | |
- | + | ||
- | **{{:2010:day4-2western_blot_lecture.pdf|Lec 2 - Immuno (Western) Blotting}} (S. Sathiasothy)** | + | |
- | + | ||
- | **[[Friday May 7]]** | + | |
- | + | ||
- | ** {{:2010:2010day5scanningelectronmicroscopy.pdf|Lec 1 - Scanning Electron Microscopy}} (Klaus Schultes)** | + | |
- | + | ||
- | ** {{:2010:day5-2-cell_culture.pdf|Lec 2 - Cell Culturing Techniques}} (Alison Cowie)**{{:2010:day8-1_mobix_talk_2010.pdf|}} | + | |
- | + | ||
- | **[[Monday May 10]]** | + | |
- | + | ||
- | Rotation exercises-- HPLC, EM, PCR, Microtiter plate, autoclave (no notes) | + | |
- | + | ||
- | **[[Tuesday May 11]]** | + | |
- | + | ||
- | Rotation exercises completion and bacterial growth curve for induction of Taq | + | |
- | + | ||
- | **[[Wednesday May 12]]** | + | |
- | + | ||
- | **Lec 1 - DNA synthesis and PCR (Alison Cowie)** | + | |
- | + | ||
- | === 2009 Lecture Notes === | + | |
- | + | ||
- | (these are maintained for students who wished to gain an overview of the course--lectures will be updated with changes (usually minor) on a daily basis as the course progresses in 2010). | + | |
- | + | ||
- | + | ||
- | 2009 | + | |
- | + | ||
- | **[[Monday, May 4]]** | + | |
- | + | ||
- | + | ||
- | **{{:presentations:techniquesin_molecular_geneticsintro.pdf|Introduction}}** (H. Schellhorn) | + | |
- | + | ||
- | **{{:presentations:primer_design_using_vector_nti.pdf|Primer design using Vector NTI}}** (T. Dong) | + | |
- | + | ||
- | **{{:presentations:records.pdf|Lab records}}** (H. Schellhorn) | + | |
- | + | ||
- | + | ||
- | **[[Tuesday, May 5]]** | + | |
- | + | ||
- | **{{:presentations:proteinoverexpressionandsigmafactorsday_2b.pdf|Protein Over Expression (Sigma Factors)}}** (H. Schellhorn) | + | |
- | + | ||
- | **{{:presentations:electrophoresis2009.pdf|Polyacrylamide Gel Electrophoresis}}** (H. Schellhorn) | + | |
- | + | ||
- | **{{:presentations:pageandhistagday_2b.pdf|Polyacrylamide Gel Electrophoresis(modified abit)}}** (H. Schellhorn) | + | |
- | + | ||
- | **[[Wednesday, May 6]]** | + | |
- | + | ||
- | **{{:presentations:2009day3cellfractionation.pdf|Cell Fractionation}}** (H. Schellhorn) | + | |
- | + | ||
- | **{{:presentations:2009day3spectroscopyenzymeassayandprotein.pdf|Spectroscopy Enzyme Assay and Protein}}** (H. Schellhorn) | + | |
- | + | ||
- | **[[Thursday, May 7]]** | + | |
- | + | ||
- | **{{:presentations:2009day4immunologymethodsandwestern.pdf|Immunological Methods}}** (H. Schellhorn) | + | |
- | + | ||
- | + | ||
- | **{{:presentations:2009day4western_blot_lecture.pdf|Western Blotting and Analysis}}** (Sharmila) | + | |
- | **[[Friday May 8]]** | + | {{:2013:day_2-2-protein_expression_electrophoresis_his_tag_purification.pdf|}} |
- | **{{:presentations:2009day5proteinenzymeassay.pdf|Protein Assays and Calculations}}** (H. Schellhorn) | + | Wednesday May 8 |
- | **{{:presentations:2009day5scanningelectronmicroscopy.pdf|Scanning Electron Microscopy }}** (Klaus Schultes) | + | {{:2013:day_3-1-cellfractionation.pdf|}} |
- | **{{:4xx3_cell_culture.pdf|Cell Culture Techniques}}** (Alison Cowie) | + | {{:2013:day_3_-_2_-_spectroscopyandenzymeassays.pdf|}} |
- | Monday May 11 | + | Thursday May 9 |
+ | {{:2013:day_4-1_immunologymethodsandwestern.pdf|}} | ||
+ | {{:20313:generalcalculationsheet4xx3_ver4.xls|General Calculation Spreadsheet for Enzymes}} | ||
- | Tuesday May 12 | + | Friday May 10 |
- | **{{:presentations:2009day7scientificmethod.pdf|Scientific Method}}** (Dr. R.A. Morton) | + | {{:2013:day_5-1-protein_enzymeassays.pdf|}} |
- | **{{:presentations:2009day7bgraphing.pdf|Publication Quality Information}}** (H. Schellhorn) | ||
- | **{{:presentations:taq_purification_procedure.ppt|Taq Purification Notes}}** (T. Dong) | + | [[Previous Years...]] |
- | **{{:mobix_talk_2009.pdf|Oligo Synthesis and sequencing}}** (A. Cowie) | ||
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