McMaster Summer Workshop in Molecular Genetics

The McMaster Workshop in Molecular Genetics began in 2000 as an applied learning experience for Biology students in their third-year of study. It was designed to equip students with the theoretical background and practical training in several key molecular biology techniques used in modern research biology labs. The format of the course, given in all day sessions over a two-week period, allow students to focus on active learning of research-based methodology without the distraction of other, concurrent courses.

This Wiki is meant to be a student-focused resource that allows easy access of critical information needed in the laboratory. Some parts of the website require a user id and password. These are automatically assigned to current students in the course but may be given upon written request by the instructor of the course.

Class of 2013 Pictures

To be added at the end of class….

Safety Training must be completed on your own schedule

Complete the Fire Safety and BSL1 non-hospital online quizzes located at BSL1 http://www.mcmaster.ca/biosafety/biosafety_training_bsl1.htm Fire Safety http://cll.mcmaster.ca/eohss/firesafety.htm

Monday May 6 Design a PCR Primer

Tuesday May 7 Prepare a publication quality graph

Wednesday May 8 Fill out an order form

Thursdday May 9 Fill out a chemical waste label

Friday May 10 Read paper and write a summary Monday May 13 Real Time PCR Analysis

Tuesday May 14 Read Assigned paper

Wednesday May 15 Fill out DNA sequencing order

Thursday May 16 Write an SOP

Lecture Note pages The notes describe the theory the experimental procedures are based on….

Scientific Supply Company page These are equipment and reagent suppliers

Manuals and Brochures Descriptions of equipment used in the course

Lab Safety Important–please review this information. During the course, we will discuss specific issues.

Chemicals used in the course (This link leads to Student-generated content…)

Tutorials

Free Software Page These are software packages that HES has used and recommends.

Shareware Page

Class Photos

Useful Books and Reference Material

Beadle G. W. and Tatum E. L. 1941 Genetic control of biochemical pathways inNeurospora.Proc. Natl. Acad. Sci. USA 27, 499–506.

Gilbert W, Maxam A (1973). “The nucleotide sequence of the lac operator”. Proc. Natl. Acad. Sci. U.S.A. 70 (12): 3581–4. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4587255

Griffin BE, Fried M, Cowie A. Polyoma DNA: a physical map (1972). Proc Natl Acad Sci U S A. 1974 May;71(5):2077-81.http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4365585

Hershey A. D. and Chase M. 1952 Independent functions of viral proteins and nucleic acid in growth of bacteriophage.J. Gen. Physiol. 36, 39–56.

Meselson M., Stahl F. W. and Vinograd J. 1957 Equilibrium sedimentation of macromolecules in density gradients.Proc. Natl. Acad. Sci. USA 43, 581–588.

Meselson M. and Stahl F. W. 1958 The replication of DNA inEscherichia coli.Proc. Natl. Acad. Sci. USA 44, 671–682.

Schrödinger E. 1944. What is life?. Cambridge University Press, Cambridge.

Summer 2010 May 3-14th

Summer 2009 May 4-15th