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group_3_presentation_1_-_next_generation_sequencing [2016/01/29 23:39] laihl |
group_3_presentation_1_-_next_generation_sequencing [2018/01/25 15:18] (current) |
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| ===== What is NGS? ===== | ===== What is NGS? ===== | ||
| - | Technological advances in molecular genetics at the end of the twentieth century have established a strong foundation for genetic analysis. Shotgun sequencing has allowed for the sequencing of longer DNA sections, which has played a significant role in the Human Genome Project (cite). In this method, DNA is enzymatically broken into smaller fragments and cloned for individual sequencing prior to realignment (cite). Nearly a decade later, subsequent advances in next generation sequencing (NGS) technologies have made genomic analysis much more economically feasible and have thus enabled applications of genomics across clinical and research settings (cite). | + | Technological advances in molecular genetics at the end of the twentieth century have established a strong foundation for genetic analysis. Shotgun sequencing has allowed for the sequencing of longer DNA sections, which has played a significant role in the Human Genome Project.<sup>1</sup> In this method, DNA is enzymatically broken into smaller fragments and cloned for individual sequencing prior to realignment.<sup>1</sup> Nearly a decade later, subsequent advances in next generation sequencing (NGS) technologies have made genomic analysis much more economically feasible and have thus enabled applications of genomics across clinical and research settings.<sup>2</sup> NGS presents a platform for high-throughput sequencing of DNA.<sup>2</sup> There are various NGS technologies, namely 454 Life Sciences, Illumina, Biosystems/SOLiD and Ion Torrent which have allowed for the sequencing of whole genomes in a cost and time efficient manner.<sup>2</sup> |
| ===== Different Types of NGS Technology ===== | ===== Different Types of NGS Technology ===== | ||
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| {{ youtube>large:nlvyF8bFDwM }} | {{ youtube>large:nlvyF8bFDwM }} | ||
| - | Figure 4 - A summary of SOLiD sequencing steps are illustrated in this video. | + | <style center>Figure 4 - A summary of SOLiD sequencing steps are illustrated in this video. </style> |
| ==== Illumina Sequencing Technology ==== | ==== Illumina Sequencing Technology ==== | ||
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| {{ :screen_shot_2016-01-28_at_2.30.21_pm.png?400 }} | {{ :screen_shot_2016-01-28_at_2.30.21_pm.png?400 }} | ||
| - | Figure 9 - A table comparing the next generation sequencing techniques (Liu, 2012). | + | <style center> Figure 9 - A table comparing the next generation sequencing techniques (Liu, 2012). </style> |
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| ===== References ===== | ===== References ===== | ||
| - | 1. | + | 1. Wilson, B. J., & Nicholls, S. G. (2015). The Human Genome Project, and recent advances in personalized genomics. //Risk Management and Healthcare Policy//, //8//, 9–20. |
| - | 2. | + | 2. Grada, A., & Weinbrecht, K. (2013). Next-generation sequencing: methodology and application. //Journal of Investigative Dermatology//, //133//(8), e11. |
| 3. Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., … Rothberg, J. M. (2005). Genome Sequencing in Open Microfabricated High Density Picoliter Reactors. //Nature//, //437//(7057), 376–380. | 3. Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., … Rothberg, J. M. (2005). Genome Sequencing in Open Microfabricated High Density Picoliter Reactors. //Nature//, //437//(7057), 376–380. | ||
