https://wiki.mcmaster.ca/Biophotonics/ Thu, 05 Nov 2026 17:29:52 -0500 FeedCreator 1.7.2-ppt DokuWiki https://wiki.mcmaster.ca/Biophotonics/lib/images/favicon.ico https://wiki.mcmaster.ca/Biophotonics/ https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:fall_2012?rev=1357672785&do=diff 2012 Plan Schedule September October November December Spring Summer 0310172401081522290512192603101724------------------------Streak Camera Configuration 1Design drift reference fiber 2Check distortion of streak camera 3Implement new 2D-1D fiber array 4Implement drift reference fiber Galvo Scanner 1Set up galvo scanner system 2Int… people:tsikourasa Tue, 08 Jan 2013 14:19:45 -0500 https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:plan?rev=1373306038&do=diff HCS-FLIM The goal for the end of the summer is to achieve a full working prototype system that will be capable of raster-scanning a fixed sample at a high speed and reconstruct a FLIM image. Improvements of the project would be: * optical system: updated to include reference fiber and window galvo scanner * reference fiber: display of reference spots on top of streak images, capable of real-time drift correction and time-axis calibration * window galvo scanner: working in raster scanni… people:tsikourasa Mon, 08 Jul 2013 13:53:58 -0500 https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:planningstage?rev=1371660772&do=diff Reference Fiber Optics Letter Literature Search H. Li et al, Improving the precision of fluorescence lifetime measurement using a streak camera, Chinese Optics Letters 8(10), 934-936 (2010). An etalon of known timing separation is used to calibrate the time axis of a streak camera. The time axis is fit accordingly to a 4th-order polynomial equation. This performs the same method of fitting non-linearity on the time axis and non-uniformity of pixel gain, but without assuming a sinusoidal patt… people:tsikourasa Wed, 19 Jun 2013 12:52:52 -0500 https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:spring_2013?rev=1357672648&do=diff 2012 Plan Schedule January February March April Summer 0714212804111825041118250108152229------------Streak Camera Configuration 1Implement 2D-1D fiber array 2Characterize readout camera 3Implement readout camera in scan code Reference Fiber Bundle … people:tsikourasa Tue, 08 Jan 2013 14:17:28 -0500 https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:summer_2011?rev=1357672847&do=diff Summer 2011 Plan Schedule May June July August 0613202703101724010815222905121926Optics Letter 1First draft of optics letter 2Perform FLIM-FRET imaging 3First round of reviews 4Second round of reviews Streak Camera 1Research input optics 2Place order for input optics 3Replace input optics 4Send streak camera to… people:tsikourasa Tue, 08 Jan 2013 14:20:47 -0500 https://wiki.mcmaster.ca/Biophotonics/people:tsikourasa:summer_2014?rev=1399303303&do=diff Summer 2014 Plan Schedule May June July August Fall 0512192602091623300714212804111825------------System Alignment 1 Align optical setup 2 Calibrate acquisition procedure 3 Finalize reconstruction procedure Live Cell Imaging 1 Perform live cell experiment comparison 2 High-con… people:tsikourasa Mon, 05 May 2014 11:21:43 -0500