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group_1_ta_karla_natstructmolbiol_2021_28_8_presentation_2 [2023/03/02 19:12] ahmads78 |
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| The C-terminal of MlaF is longer than its homolog, LptB, and the extra residues of MlaF allow it to interact with MlaB and the other MlaF subunit. It was determined that even though the C-terminal is not involved in the ATPase or transport function of the overall complex, it plays a key role in stabilizing the complex as it could not be purified when the C-terminal was deleted. It was also found that S146A and R151A substitutions truncated PL transport and cell survival when chlorpromazine was introduced (Extended figure 3). Overall, the authors found that ATP hydrolysis may be required for Mla complex conformational change to occur. | The C-terminal of MlaF is longer than its homolog, LptB, and the extra residues of MlaF allow it to interact with MlaB and the other MlaF subunit. It was determined that even though the C-terminal is not involved in the ATPase or transport function of the overall complex, it plays a key role in stabilizing the complex as it could not be purified when the C-terminal was deleted. It was also found that S146A and R151A substitutions truncated PL transport and cell survival when chlorpromazine was introduced (Extended figure 3). Overall, the authors found that ATP hydrolysis may be required for Mla complex conformational change to occur. | ||
| - | <box 35% round right | > | + | ===== Limitations and Future Directions ===== |
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| {{ :figure5a_b.png?nolink400|}} | {{ :figure5a_b.png?nolink400|}} | ||
| </box| Figure 4a,b:Residues binding ADP (a) or AMP-PNP (b) and Mg2+ ions in the catalytic site of one MlaF subunit and the signature motif of the other MlaF subunit containing conserved residues Glu 144, Ser 146 and Arg 151.> | </box| Figure 4a,b:Residues binding ADP (a) or AMP-PNP (b) and Mg2+ ions in the catalytic site of one MlaF subunit and the signature motif of the other MlaF subunit containing conserved residues Glu 144, Ser 146 and Arg 151.> | ||
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| - | ===== Limitations and Future Directions ===== | ||
| In terms of general critiques, Tang et al. lacked in the organization of the paper. The results are not communicated effectively to the readers, which is especially present with the figures and figure captions. The figures are scattered across the paper, making it difficult to connect the results mentioned to supplementary data. For example, the caption for Figure 1 is on a separate page from the figure itself. This makes the paper appear messy and hinders the overall organization, resulting in an overall incoherent read. In addition, the general information provided in the paper does not flow well, and sufficient background information in order to understand the results was lacking. | In terms of general critiques, Tang et al. lacked in the organization of the paper. The results are not communicated effectively to the readers, which is especially present with the figures and figure captions. The figures are scattered across the paper, making it difficult to connect the results mentioned to supplementary data. For example, the caption for Figure 1 is on a separate page from the figure itself. This makes the paper appear messy and hinders the overall organization, resulting in an overall incoherent read. In addition, the general information provided in the paper does not flow well, and sufficient background information in order to understand the results was lacking. | ||
