===== 2012 Plan ===== ==== Schedule ==== ^ ^ ^ September ^^^^ October ^^^^^ November ^^^^ December ^^^^ Spring ^ Summer ^ ^ ^ ^03^10^17^24^01^08^15^22^29^05^12^19^26^03^10^17^24^------------^------------^ |**Streak Camera Configuration** ||||||||||||||||||||| |**1**|Design drift reference fiber ^ || || ||||| |||| |||| | | |**2**|Check distortion of streak camera | |^ || ||||| |||| |||| | | |**3**|Implement new 2D-1D fiber array | ||^ | ||||| |||| |||| | | |**4**|Implement drift reference fiber | |||| |||^ | |||| |||| | | |**Galvo Scanner** ||||||||||||||||||||| |**1**|Set up galvo scanner system | |||| ||||| ||^ || ||| | | |**2**|Interface with galvo scanner | |||| ||||| |||| ^ | || | | |**3**|Test scanner performance | |||| ||||| |||| |^ || | | |**4**|Implement scanner in system | |||| ||||| |||| |||^ | | |**Beam Shaper** ||||||||||||||||||||| |**1**|Research simple beam shapers | |||^ || ||| |||| |||| | | |**2**|Decide on beam shaper | |||| |^ || | |||| |||| | | |**3**|Implement beam shaper | |||| ||||| |^ | | |||| | | |**Readout Camera** ||||||||||||||||||||| |**1**|Research high speed readouts | |||| ||||^ || || |||| | | |**2**|Decide on high speed readout | |||| ||||| |^ || |||| | | |**3**|Interface readout camera | |||| ||||| |||| |||^ | | |**4**|Test readout performance | |||| ||||| |||| |||^ | | |**5**|Implement readout in system | |||| ||||| |||| |||| ^ | |**Live Cell Imaging** ||||||||||||||||||||| |**1**|Perform FLIM-FRET experiments | |||| ||||| |||| |||^ | | |**2**|Reconstruct FLIM images | |||| ||||| |||| |||^ | | |**3**|Write journal article on results | |||| ||||| |||| |||| ^ | |**Image Reconstruction** ||||||||||||||||||||| |**1**|Write robust lifetime estimation code | |||| ||||| |||| |||^ | | |**2**|Extend to multi-exponential | |||| ||||| |||| |||^ | | |**3**|Interpret FRET efficiency | |||| ||||| |||| |||^ | | /* |**Streak Camera** ||||||||||||||||||| |**1**|Research input optics | |||^ || ||| |||| |||| |**2**|Place order for input optics | |||| ^ | ||| |||| |||| |**3**|Replace input optics | |||| |^ ||| |||| |||| |**4**|Send streak camera to Optronis | |||| |||^ | |||| |||| |**Scanning System** ||||||||||||||||||| |**1**|Perform proof of principle | ||^ || |||| |||| |||| |**2**|Research available products | |||^ ||| || |||| |||| |**3**|Place order for chosen product | |||| |^ | | ||||| |||| |**4**|Align solution to system | |||| |||| ^ || || |||| |**5**|Write program for scan | |||| |||| ||^ | | |||| |**6**|Reconstruct image | |||| |||| |||^ | |||| |**Readout Camera** ||||||||||||||||||| |**1**|Research readout cameras | |||| |||^ || ||| |||| |**Photon Efficiency** ||||||||||||||||||| |**1**|Acquire higher power laser head | ||^ | |||| ||||| |||| |**2**|Research more efficient filter | |||| |||| ||||^ || ||| */ \\ ==== Milestones ==== ^**September 28**|Implementation of new 2D-1D fiber array| ^**November 2**|System is correctable for drift, distortion| ^**November 23**|Clean uniform excitation light from beam shaper| ^**Spring 2012**|Implementation of galvo scanner| ^**Spring 2012**|in vivo FLIM imaging results| ^**Summer 2012**|Implementation of high-speed readout camera|