===== 2011 Summer Plans ===== \\ **Goal:** Finish FLIM project and finalized the results\\ \\ **Main purpose of the study:** \\ Characterizing time-lapse fluorescence lifetimes of Photofrin at different intracellular locations in order to realize if FLIM can be a potential real-time dose metric for PDT.\\ \\ ---- === May === **5 / 4 - 5 / 10** :\\ Restart the cell culture and data analysis of (L1_1.5hr to 4 hr)\\ \\ **5 / 11 - 5 / 17** :\\ Data analysis (Cells are getting happier during these two weeks)\\ \\ **5 / 18 - 5 / 24** :\\ Start FLIM experiment using SP5 \\ * Low concentration (5ug/ml), 1 hour of incubation (need 2 more replicates)\\ * Data analysis\\ \\ **5 / 25 - 5 / 31** :\\ FLIM experiment (cont'd)\\ * Low concentration (5ug/ml), 1.5 hr to 4 hr of incubation (need 2 more replicates)\\ * Data analysis\\ \\ ---- === June === **6 / 1 - 6 / 10**:\\ FLIM experiment and organize all results (low concentration)\\ * Low concentration (5ug/ml), 18 hr of incubation -- need 2 more replicates * Data analysis\\ \\ **6 / 11 - 6 / 30**:\\ Organize all results using low concentration Photofrin\\ * Decide what other measurements need to be done (i.e. calibration of IRF..etc) after summarizing the results.\\ * Note: We don't have any repetitive measurement for high concentration photofrin (20 ug/ml), not sure if it's necessary \\ \\ ---- === July === **7 / 1 - 7 / 30**:\\ to be arranged based on experimental results and due date\\ * May start to finalize FLIM project, hopefully will be able to start the manuscript preparation (i.e. write-up of some results and methods). Angela may perform some leftover experiments.\\ \\ ---- === August === Hopefully working on the manuscript of FLIM project. To be arranged based on the progress. \\