2010

**[[Monday May 3]]**

**{{:2010:day_1-1-introduction.pdf|Lec 1 - Introduction&LabRecords}} (H.E. Schellhorn)**

**{{:2010:day_1-2-proteinexpression_.pdf|Lec 2 - Proteins&SDS}} (H.E. Schellhorn)**

**[[Tuesday May 4]]**

**{{:2010:day_2-1-primer_design_using_vector_nti.pdf|Lec 1 - DNA primer design and software}} (T.Dong)**

**{{:2010:day_2-2-electrophoresis.pdf|Lec 2 - PAGE separation}} (S. Chiang)**

**[[Wednesday May 5]]**

**{{:2010:day3-2-cellfractionation.pdf|Lec 1 - CellFractionation}} (H.E Schellhorn)**

**{{:2010:day_3-2-spectroscopyandenzymeassays.pdf|Lec 2 - SpectroscopyandEnzymeAssays}} (H.E Schellhorn)**

**[[Thursday May 6]]**

**{{:2010:day4-1-protein_enzymeassays.pdf|Lec 1 - Protein&EnzymeAssays}} (H.E. Schellhorn)**

**{{:2010:day4-2western_blot_lecture.pdf|Lec 2 - Immuno (Western) Blotting}}  (S. Sathiasothy)**

**[[Friday May 7]]**

** {{:2010:2010day5scanningelectronmicroscopy.pdf|Lec 1 - Scanning Electron Microscopy}} (Klaus Schultes)**

** {{:2010:day5-2-cell_culture.pdf|Lec 2 - Cell Culturing Techniques}} (Alison Cowie)** 

**[[Monday May 10]]**

Rotation exercises-- HPLC, EM, PCR, Microtiter plate, autoclave (no notes)

**[[Tuesday May 11]]**

Rotation exercises completion and bacterial growth curve for induction of Taq

**[[Wednesday May 12]]**

**{{:2010:day8-1_mobix_talk_2010.pdf|Lec 1 - DNA synthesis and PCR}}   (Alison Cowie)**

**[[Thursday May 13]]**

**{{:2010:day_9-fluorescence.pdf|Lec 1 - Immunofluorescence and Fluorescent Proteins}}  (H.E. Schellhorn)**

=== 2009 Lecture Notes ===

(these are maintained for students who wished to gain an overview of the course--lectures will be updated with changes (usually minor) on a daily basis as the course progresses in 2010).


 2009

**[[Monday, May 4]]**


**{{:presentations:techniquesin_molecular_geneticsintro.pdf|Introduction}}** (H. Schellhorn)

**{{:presentations:primer_design_using_vector_nti.pdf|Primer design using Vector NTI}}** (T. Dong)

**{{:presentations:records.pdf|Lab records}}** (H. Schellhorn)


**[[Tuesday, May 5]]**

**{{:presentations:proteinoverexpressionandsigmafactorsday_2b.pdf|Protein Over Expression (Sigma Factors)}}** (H. Schellhorn)

**{{:presentations:electrophoresis2009.pdf|Polyacrylamide Gel Electrophoresis}}** (H. Schellhorn)

**{{:presentations:pageandhistagday_2b.pdf|Polyacrylamide Gel Electrophoresis(modified abit)}}** (H. Schellhorn)

**[[Wednesday, May 6]]**

**{{:presentations:2009day3cellfractionation.pdf|Cell Fractionation}}** (H. Schellhorn) 

**{{:presentations:2009day3spectroscopyenzymeassayandprotein.pdf|Spectroscopy Enzyme Assay and Protein}}** (H. Schellhorn) 

**[[Thursday, May 7]]**

**{{:presentations:2009day4immunologymethodsandwestern.pdf|Immunological Methods}}** (H. Schellhorn)

 
**{{:presentations:2009day4western_blot_lecture.pdf|Western Blotting and Analysis}}** (Sharmila) 

**[[Friday May 8]]**

**{{:presentations:2009day5proteinenzymeassay.pdf|Protein Assays and Calculations}}** (H. Schellhorn)

**{{:presentations:2009day5scanningelectronmicroscopy.pdf|Scanning Electron Microscopy }}**   (Klaus Schultes)

**{{:4xx3_cell_culture.pdf|Cell Culture Techniques}}** (Alison Cowie)

Monday May 11

Tuesday May 12

**{{:presentations:2009day7scientificmethod.pdf|Scientific Method}}**     (Dr. R.A. Morton)

**{{:presentations:2009day7bgraphing.pdf|Publication Quality Information}}**  (H. Schellhorn)

**{{:presentations:taq_purification_procedure.ppt|Taq Purification Notes}}**  (T. Dong)

**{{:mobix_talk_2009.pdf|Oligo Synthesis and sequencing}}** (A. Cowie)