====== MANUALS AND BROCHURES ======

The following resource shows the equipment we will be using along with associated manuals and documents...

=====PROCEDURES====

**Protein Assays **
**Biorad Bradford Protein Assay kit **
//[[http://www.bio-rad.com/LifeScience/pdf/Bulletin_1069.pdf|     Brochure]]
[[http://www.bio-rad.com/LifeScience/pdf/Bulletin_9004.pdf|     Manual]]//

{{:prot-assay_web.jpg|}}

**Protein Purification**
**Purification of Protein – His-tag**
Agarose Bead Technologies
//[[http://www.abtbeads.com|    for other manuals]]
[[http://www.abtbeads.com/literature/purification/his-tag/selection_criteria_purification.pdf|     Brochure]]
[[http://www.abtbeads.com/literature/purification/his-tag/procedures/chelates_spin_procedure.pdf|     Manual]]
//

{{:agarose_bead_technologies.jpg|}}

===== EQUIPMENT =====

**Liquid Handling**
**Gilson Pipetman **
//[[http://www.gilson.com/PDFs/pdfid29.pdf|Brochure]]
[[http://www.unh.edu/instrumentation-center/PipetteCareandMaintenance.pdf|     Manual]]//

{{:pipettetips.jpg|}}{{:pipettemanimages.jpg|}}

**Polymerase Chain Reaction**
Eppendorf Mastercycler Gradient PCR cycler 
//[[http://www.eppendorf.ca/script/binres.php?RID=36322&DOW=1&pb=776b3d2dc5187f1a|     Brochure]]
[[http://www.eppendorf.ca/script/binres.php?RID=36322&DOW=1&pb=776b3d2dc5187f1a|     Manual]]//

{{:gradientpcr2.jpg|}}

**Polymerase Chain Reaction**
**Biorad Mycycler Thermal Cycler**
//[[http://www.bio-rad.com/cmc_upload/Literature/52166/Bulletin_2855F.pdf|     Brochure]]
[[http://www.bio-rad.com/cmc_upload/Literature/52004/4006219F.pdf|     Manual]]//

{{:mycycler2.jpg|}}

**Protein Electrophoresis Equipment**
***Biorad Protean TetraCell**
//[[http://www.bio-rad.com/cmc_upload/Literature/206070/Bulletin_5535.pdf|     Brochure]]
[[http://www.bio-rad.com/cmc_upload/Literature/37363/4006158B.pdf |     Manual (Quick Guide)]]
[[http://www.bio-rad.com/cmc_upload/Literature/44432/4006157B.pdf|     Manual (Detailed)]]//

{{:tetra_cell_system.jpg|}}

[[http://bio-rad.centra.com/bio-radroot/learning%20objects/mini-protean_tetra/mini-protean_tetra_cell.htm|TUTORIAL]]

**Protein Detection**
**Biorad SemiDry Transfer unit **
//[[http://www.bio-rad.com/LifeScience/pdf/Bulletin_1714.pdf|     Brochure]]
[[http://www.bio-rad.com/cmc_upload/Literature/12490/M1703957B.pdf|      Manual (DNA and RNA)]]
//

{{:trans-blot_sd_semi-dry_transfer_cel.jpg|}}

**Protein Detection**
**Biorad  Transblot **
//[[http://www.bio-rad.com/cmc_upload/0/000/013/280/M1703930E.pdf|      Manual- Transblot]]//

{{:transblot.jpg|}}

**Protein and Metabolite Separation**
**High Performance Liquid Chromatography (HPLC) **
Agilent 1200 Series HPLC
//[[http://www.chem.agilent.com/en-US/Products/Instruments/lc/1200seriesrapidresolutionlcsystem/Pages/default.aspx|     Website]]
[[http://www.chem.agilent.com/en-us/Search/Library/_layouts/Agilent/PrimaryDocumentViewer.ashx?whid=55631&liid=1022|     Brochure]]
[[http://www.chem.agilent.com/Library/usermanuals/Public/G1312-90300_RRLC_System_Manual_ebook.pdf|      Manual]]
//

{{:agilent_1200_series_hplc_.jpg|}}

**Spectrophotometry**
**Spectrophotometer**
Thermo Scientific Genesys
//[[http://www.thermo.com/eThermo/CMA/PDFs/Product/productPDF_3560.pdf |     Brochure]] // 
Manual

{{:thermo_scientific_genesys.jpg|}}

**Spectrophotometry **
**MicrotiterPlate Reader**
Thermo Multiskan Microtiter Plate reader
//[[http://www.thermo.com/eThermo/CMA/PDFs/Various/File_4715.pdf|     Brochure]]//
Manual

{{:multiskan_microtiter_plate_reader.jpg|}}